Fig. 4

Signaling pathway associated with CHAF1A. A: Volcano plot for differentially expressed genes (DEGs) between high and low subgroup of CHAF1A expression in the AHJU cohort. B: Heatmap of the TOP 40 DEGs. C: Enrichment analysis for KEGG signaling pathway associated with CHAF1A. D: The ROC curves for MSI prediction by CHAF1A mRNA expression. E: MSI incidence according to CHAF1A expression level. Based on the optimal threshold of CHAF1A expression for the maximum ROC curve values, the patients are dichotomized into high and low subgroups. F: The ROC curves for TMB-high prediction by CHAF1A expression. The optimal cutoff value with the most significant survival difference was used to to define high and low TMB. G: TMB-high incidence according to CHAF1A expression level. AHJU Affiliated Hospital of Jiangsu University, KEGG Kyoto Encyclopedia of Genes and Genomes, MSI microsatellite instability, TMB tumor mutation burden, ROC receiver operating characteristic curve, AUC the areas under the ROC curves