The spike-specific TCRβ repertoire shows distinct features in unvaccinated or vaccinated patients with SARS-CoV-2 infection

Table 4 Summary of TCRβ associated with spike peptides. In bold, the data represented in Fig. 5b are highlighted

aa Position^a	Unvaccinated			Vaccinated			p value^e
aa Position^a	n. clonotypes^b	Median enrichment^c	Median clonal fraction^d	n. clonotypes	Median enrichment	Median clonal fraction	p value^e
23–59	55	15.8	0.0063	13	8.8	0.0162	0.1589
82–94	9	12,1	0.0115	73	15.0	0.0203	0.3630
135–177	20	11.3	0.0015	11	9.8	0.0222	0.0116
207–240	10	8.4	0.0021	9	8.8	0.0159	0.1834
264–276	16	19.8	0.0025	5	8.4	0.0261	< 0.0001
319–350	118	11.2	0.0051	108	8.8	0.0203	< 0.0001
385–423	41	16.5	0.0039	58	8.6	0.0187	0.7000
448–472	30	8.7	0.0039	26	10.3	0.0194	< 0.0001
488–506	21	8.8	0.0094	10	9.3	0.0296	0.071
582–591	1	8.0	0.0027	0			–
645–645	5	17.9	0.0062	0			–
673–699	24	15.5	0.0041	29	15.5	0.0004	0.0013
705–732	25	18.2	0.0191	17	8.7	0.0156	0.6349
751–760	2	9.6	0.0084	0			–
778–792	0			3	9.9	0.0070	–
859–864	16	8.8	0.0039	16	8.8	0.0050	0.2016
893–902	0			2	8.4	0.0120	-
986–999	18	8.6	0.0088	12	8.9	0.0176	0.2381
1015–1027	15	74.2	0.0352	14	10.3	0.0266	0.6230
1046–1059	21	15.3	0.0074	16	13.2	0.0061	0.1289
1260–1269	0			1	14.7	0.012	–

^aAmino acid position in the spike protein (uniprot ID P0DTC2)
^bNumber of clonotypes associated to epitope within the indicated spike region; showing a post/pre frequency > 8
^cMedian of the enrichment (post/pre stimulation ratio of clonal fration) showed by the clonotypes associated to epitope within the indicated spike region
^dMedian of the pre-stimulation clonal fration showed by the clonotypes associated to epitope within the indicated spike region
^eStatistical differences were evaluated by Mann–Whitney test

ISSN: 1479-5876