Fig. 5

Parkin mini-peptide conjugates rescue altered mitochondrial ROS levels in Parkin-deficient cells. A, B Delivery of mini-peptide-conjugates #1 (iii), #3 (iv), #4 (v) reduce mitochondrial ROS production in Parkin KD SH-SY5Y cells (ii). (i) WT SH-SY5Y. Statistical differences were calculated by one-way ANOVA followed by Holm-Sidak post hoc test to correct for multiple comparisons ****p ≤ 0.0001. Scale bar: 30 μm. C, D Mitochondrial ROS production is reduced by mini-peptides #3 (iv) and #4 (v) in hiPSC-derived neurons carrying a PRKN mutation (PRKN NT, not treated, ii). (i) hiPSC-derived neurons of a control individual; (iii) mini-peptide #1 in hiPSC-derived neurons carrying a PRKN mutation. Scale bar: 50 μm. Statistical differences were calculated by one-way ANOVA followed by Holm-Sidak post hoc test to correct for multiple comparisons ***p ≤ 0.001, *p ≤ 0.05