Fig. 4

Parkin RING0 expression rescues altered mitochondrial phenotypes in Parkin-deficient cells. A Maximum respiratory capacity (ETS) in Parkin knockdown (KD) SH-SY5Y cells was significantly rescued after overexpression of Parkin RING0 and full-length Parkin (Parkin WT). Statistical differences were calculated by one-way ANOVA followed by Tukey's post hoc test to correct for multiple comparisons *p ≤ 0.05. B Complex I—dependent respiration was significantly restored after overexpression of Parkin RING0 and Parkin WT in Parkin KD SH-SY5Y cells. Statistical differences were calculated by one-way ANOVA followed by Tukey’s post hoc test to correct for multiple comparisons *p ≤ 0.05, **p ≤ 0.01. C Mitochondrial ROS levels were significantly reduced after overexpression of Parkin RING0 (iii), RING0 conjugated with a mitochondrial leading sequence (iv), and Parkin WT (v) in Parkin KD (ii) SH-SY5Y cells. WT SH-SY5Y cells are shown in panel (i). MitoSox Red mitochondrial superoxide staining is shown in red. Scale bar: 50 µm. Statistical differences were calculated by one-way ANOVA followed by Tukey’s post hoc test to correct for multiple comparisons ****p ≤ 0.0001