Fig. 8

PRR knockdown blunted pyroptotic cell death and fibrotic response in db/db mice via DPP4-mediated signaling. A–F The protein levels of NLRP3, cleaved-Caspase1, GSDMD-N, IL-1β, IL-18 were assessed by Western blot. Representative Western blot (A) and quantitative data (B–F) are showed (n = 6). C Representative immunofluorescence microscopy images of coimmunostaining PRR (red) with GSDMD (green) in renal sections from WT and db/db mice with or without PRR ablation. Bar = 20 μm. H and I Elisa detection of urinary IL-1β (H) and IL-18 (I) concentration, the quantitative data were normalized with urinary creatine (n = 6). J–K western blot analyses (J) and quantitative data (K) showed the abundance of DPP4, phosphorylation level of JNK, SIRT3 and FGFR1 (n = 6). L Representative fluorescent images of renal sections from mice with or without PRR silence and stained with PRR (red), DPP4 (green). Bar = 20 μm. Data are presented as mean ± SEM of biologically independent samples. ∗ P < 0.05, ∗∗ P < 0.01. One-way ANOVA was used to analyze the data among multiple groups, followed by Tukey’s post hoc test