Fig. 3

Overexpression of PRR promotes HK-2 cell pyroptosis. (A and B) Western blot analyses. A and quantitative data (B) showed the increased protein abundance of PRR in PRR overexpression plasmid transfected HK-2 cells. (n = 3). (C and D) Flow cytometry analysis showed that active Caspase1⁺PI⁺ HK-2 cells were increased by PRR overexpression (C) and quantitative data (D) (n = 3). E PRR was overexpressed in HK-2 cells, and the Caspase1 activity in cell lysis was determined by kits (n = 5). (F and G) Western blot analyses (F) and quantitative data (G) showed that PRR overexpression increased NLRP3, cleaved-Caspase1, GSDMD-N, IL-1β and IL-18 expression in HK-2 cells. (n = 3). H and I PRR was overexpressed in HK-2 cells, and the IL-1β (H), IL-18 (I) or IL-6 (J) concentration in the culture medium was determined by ELISA, and then normalized by protein concentration in cell lysis. (n = 3). Data are presented as mean ± SEM of biologically independent samples. ∗ P < 0.05, ∗∗ P < 0.01. P values were determined by Student’s t-test