Fig. 3
Immunogenicity of selected neoantigens. A Experimental design for testing natural tumor immunogenicity against selected neoantigens. B Tumor growth in mice inoculated with 105 B16-F10 cells. Each line represents one animal, two males (dark blue) and two females (light blue) are represented. C Evaluation of the cellular response against selected neoantigens in mice inoculated with B16-F10 cells. D Experimental design for testing neoVLP immunogenicity. Blood samples were taken before each vaccination and at endpoint, and spleen was recovered at endpoint. Two vaccines were administered with a three-week interval, and all animals were euthanised two weeks after the second immunization. E–H Evaluation of cellular responses generated against the selected neoantigens. Tier1-GAG in dark red, Tier2-GAG in yellow, Tier3-GAG in purple, Frameshift-GAG in light blue and naked-VLP in grey