Fig. 3

Knockdown of NCAPG2 suppresses PCa cell migration, invasion, and regulates cell cycle of PCa cells in vitro. a Colony formation assay showed that LNCaP and DU145 cells in sh-NCAPG2 group formed fewer colonies. b, c The migration rate and invasion ability of LNCaP and DU145 cells were determined by wound healing assay and transwell assay, respectively. d–g Cell cycle and WB analysis for LNCaP and DU145 cells transfected with sh-NC or sh-NCAPG2. Both two PCa cells showed an increase in the percentage of cells arrested in G2 phase with a concomitant decrease in S phase following NCAPG2 downregulation. WB assays indicated that knockdown of NCAPG2 led to a significant increase in the expression of p21 protein. PCa prostate cancer, WB western blot. P values were calculated by the Student’s t-test. * means P < 0.05, ** means P < 0.01, *** means P < 0.001, ns means P > 0.05, and P < 0.05 is defined as statistically significant