Fig. 2

NCAPG2 is highly expressed in PCa cells and knockdown of NCAPG2 inhibits PCa cell proliferation in vitro. a, b Compared with WPMY-1 cells, levels of NCAPG2 mRNA (qPCR) and protein (WB) were highly expressed in PC-3, DU145 and LNCaP cells. c In DU145 cells, compared with the sh-NC group, the knockdown efficiency of NCAPG2 in shNCAPG2-3 group was 50.9%, whereas the shNCAPG2-1 group or shNCAPG2-2 group showed no significant change. d, e qPCR and WB analysis of NCAPG2 expression in LNCaP and DU145 cells after transfection of sh-NC or sh-NCAPG2. f–h LNCaP and DU145 cells proliferation were analyzed by Celigo cell counting and CCK-8 assay, following NCAPG2 knockdown. PCa prostate cancer, qPCR quantitative real-time polymerase chain reaction, WB western blot, CCK-8 cell counting kit-8. P values were calculated by the Student’s t-test. * means P < 0.05, ** means P < 0.01, *** means P < 0.001, ns means P > 0.05, and P < 0.05 is defined as statistically significant