Fig. 9

MiR-410-5p maintains maternal–fetal interface immune tolerance in vivo. A The expression differences of IL-23, TNF-α, IL-10, and TGF β in the pregnant uterus and placenta of mice supplemented with agomir-410-5p before LPS infection were detected using qRT-PCR. B qRT-PCR was used to detect the expression differences of IL-23, TNF-α, IL-10, and TGF β in the pregnant uterus and placenta of agomir-410-5p-supplemented mice after LPS infection. (* represents the difference in LPS- the related group compared with the naive-ctrl group, and ^# represents the difference between agomir-410-5p and agomir-NC). C IHC was used to detect the expression of CD86 proteins in the uterine tissue of mice (n = 3). D IHC was used to detect the expression of CD206 proteins in the uterine tissue of mice (n = 3). E Simple Western was used to detect the expression of CD86 proteins in the uterine tissue of mice (n = 3). F Simple Western was used to detect the expression of PPAR γ proteins in the uterine tissue of mice (n = 3). Values were listed as the mean ± SEM. Ns not significant, * P < 0·05, **P < 0·01, ***P < 0·001, ****P < 0·0001