Fig. 6

MiR-410-5p inhibits M1 macrophage polarization, regulates macrophage metabolism, and affects ROS generation by suppressing the STAT1 signal pathway. A The ECAR of M0/M1/M2 and transfected-M0 macrophages were detected using a multi-function enzyme labelling instrument. B The OCR of M0/M1/M2 and transfected-M0 macrophages were detected using a multi-function enzyme labelling instrument. C The macrophages transfected with miR-410-5p mimic, oe-STAT1 and si-STAT1 were analyzed by Simple Western with macrophage phenotypic antibodies CD86. D The macrophages transfected with miR-410-5p mimic, oe-STAT1 and si-STAT1 were analyzed by Simple Western with macrophage phenotypic antibodies CD163. E Multi-factor flow microspheres were used to detect the expression of IL-4, IL-6, IL-10, IL23, IP-10, TARC and TNF α in the medium of M0/M1/M2 and transfected-M0 macrophages. F–G The mRNA levels of CPT1a and CPT2 in M0 macrophages were detected using qRT-PCR. Values were listed as the mean ± SD. ns not significant, *P < 0·05, **P < 0·01, ***P < 0·001, ****P < 0·0001