Fig. 5

STAT1 activates macrophage M1 polarization. A The transfection efficiency of STAT1 overexpression vector(oe-STAT1) and interference vector(si-STAT1) in macrophages. B STAT1 knock-down efficiency in macrophages. C STAT1 overexpression efficiency in macrophages. D The expression differences of IL-23, TNF-α, IL-10 and TGF-β in induced-M0 macrophages by oe-STAT1 or si-STAT1 were detected by qRT-PCR. E The macrophages transfected with oe-STAT1 and si-STAT1 were analyzed by Simple Western with macrophage phenotypic antibodies (CD86, CD163, IRF5, and PPAR γ). F The contents of IL-6 and TNF α in the medium of macrophages were detected by ELISA. G The mRNA levels of glycolysis (G6PD, LDHA, HK2, HK3) in M0 macrophages were detected by qRT-PCR. H The mRNA levels of CPT1a and CPT2 in M0 macrophages were detected by qRT-PCR. (ctrl in D-H represents M0 macrophages). Values were listed as the mean ± SD. Ns not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001