Fig. 4

MiR-410-5p regulates STAT1 in vitro. A Schematic diagram of STAT1 non-coding regions and the mutation that occurred at the predicted miR-410-5p binding site. B The relative luciferase activity of macrophages transfected with WT-STAT1 + miR-NC, WT-STAT1 + miR-410-5p, MUT-STAT1 + miR-NC, and MUT-STAT1 + miR-410-5p. C Using PCR to verify the efficiency of miR-410-5p binding STAT1 RNA in miRNA pull-down experiment. D Using qRT-PCR to verify the efficiency of miR-410-5p binding STAT1 RNA in miRNA pull-down experiment. E The expression of STAT1 mRNA in macrophages transfected with miR-410-5p mimic or inhibitor was detected by qRT-PCR. F The expression of STAT1 protein in macrophages transfected with miR-410-5p mimic or inhibitor was detected by Simple Western. Values were listed as the mean ± SD. Ns not significant, *P < 0·05, ***P < 0·001, ****P < 0·0001