Fig. 3

MiR-410-5p regulates macrophage phenotype. A The protein expressions of CD68 and CD80 were analyzed using immunofluorescence in M0 macrophages. B The protein expressions of CD68 and CD163 were analyzed using immunofluorescence in M0 macrophages. C The macrophages transfected with miR-410-5p mimic and inhibitor were analyzed by Simple Western with macrophage phenotypic antibodies (CD80, CD163, IRF5, and PPARγ). D The expression differences of IL-12, IL-23, TNF α, IL-10 and Mrc1 in M0 macrophages were detected using qRT-PCR. E Multi-factor flow microspheres were used to detect the expression of IL-4, IL-6, IL-10, IL23, IP-10, TARC and TNF α in the medium of M0/M1/M2 and transfected-M0 macrophages. Values were listed as the mean ± SD. ns, not significant, *P < 0·05, **P < 0·01, ***P < 0·001, ****P < 0·0001