Fig. 3

SphK1 inhibition or ablation reduces vessel density in HCC tumors. A DEN-injected mice were treated with vehicle (veh) or PF-543 (PF) for 12 weeks. Blood vessels in non-tumorous (NT) and tumorous (T) liver tissues were stained and quantified by CD31 immunohistochemistry; scale bar = 50 μm; n = 9. B Blood vessels in DEN-injected wild-type (WT) and Sphk1 knockout (KO) mice were examined by CD31 immunohistochemistry; scale bar = 50 μm; n = 5. Data are expressed as mean ± SD. **p < 0.01; ***p < 0.001