Fig. 5
Impact of fibrosis on the migration and invasion abilities of tumour cells. A-D Following Aristolochic acid stimulation, both J82 and UMUC3 cell lines show a significant increase in WNT7B and SDC1 expression, no significant changes in LUM, VEGFA, TIMP3 expressions. E No significant changes in migration and invasion abilities in J82 and UMUC3 after Aristolochic acid stimulation. F–I After Aristolochic acid stimulation and co-culturing with fibroblasts followed by puromycin selection: significant decrease in LUM, TIMP3 expressions and a significant increase in VEGFA expression in both co-cultured cell lines, with no significant changes in WNT7B and SDC1 levels. J Significant changes in migration and invasion abilities in J82 and UMUC3 after Aristolochic acid stimulation and co-culturing with fibroblasts followed by puromycin selection. (p > 0.05 as ns, p ≤ 0.05 as *, p ≤ 0.01 as **, p ≤ 0.001 as ***, p ≤ 0.0001 as ****)