Fig. 1

ChemR23 knockout aggravated post-stroke neurological deficits, infarct volume and neuronal cell death. A, B Representative immunoblots and quantification of ChemR23 in the ipsilateral brain at 6 h, 12 h, 1 day and 3 days after MCAO (n = 3/per group). C qRT-PCR analysis of ChemR23 in the ipsilateral brain at 6 h, 12 h, 1 day and 3 days after MCAO (n = 3/per group) (D, E) Grip test and mNSS assessment of mice in each group at Day 1, Day 3, Day 7, and Day 14 after MCAO (n = 10/per group). F, G Representative images of brain slices by TTC staining and Cresyl Violet staining in each group at Day 1, Day 3, Day 7, and Day 14 after MCAO and the quantitative analysis of infarct volume (n = 6/per group). Scale bar = 5 mm. H, I Representative immunofluorescence staining images and quantification of neuronal cell death based on NeuN and TUNEL assay in the ischemic ipsilateral brain regions at Day 1 after MCAO (n = 3/per group). Scale bar = 20 μm. Data are represented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001