Fig. 5

SRSF3 interacts with has_circ_0050386 and FN1 in lung cancer cells. A Schematic of biotin-labeled hsa_circ_0050386 and NC probes used for RNA–protein pull-down in A549 cell lysates. A. Silver staining identifying proteins interacting with hsa_circ_0050386. B Venn diagram showing proteins identified by mass spectrometry and proteins predicted by catRAPID. C Mass spectrometry showing the SRSF3 peptide enriched in the hsa_circ_0050386 probe. D Western blot analysis showing protein expression of SRSF3 enriched in hsa_circ_0050386 or NC probe in A549 cell lysates. E RNA immunoprecipitation (RIP) assays together with qRT-PCR showed that SRSF3 and hsa_circ_0050386 were enriched using anti-SRSF3 antibody in A549 cell lysates. F–G. qRT-PCR and Western blot analysis showing the expression of FN1 in A549 and H1299 cells after knocking down the expression of SRSF3 in cells