Fig. 4

has_circ_0050386 down-regulates FN1 expression and inhibits lung cancer progression. A Volcano plot showing the differentially expressed genes upon overexpression of has_circ_0050386 in A549 and PC9 cells. B Venn diagram showing the set of mRNAs found to be downregulated or upregulated in both A549 and PC9 cells (filtered by fold change < -2 and adjusted P < 0.05). C Pathway enrichment of the differentially expressed genes according to KEGG analysis. D, F qRT-PCR and Western blot analysis showing relative FN1 expression in lung cancer cells after has_circ_0050386 overexpression or knockdown in cells. E Representative IHC images of FN1 in xenograft tumor tissue. Scale bar, 50 μm. G IncuCyte Live Cell Analysis System showing the viability of H1299 and PC9 cells stably transfected with shRNA-NC or shRNA 1# or co-transfected with si-NC or si-FN1. H–I A migration and invasion assay for detecting cell migration and invasion ability of H1299 and PC9 cells stably transfected with shRNA-NC or shRNA 1# or co-transfected with si-NC or si-FN1