Fig. 1

The expression and characterization of hsa_circ_0050386 in lung cancer. A volcano plot showing differentially expressed circRNAs in 8 paired lung cancer tissue samples. The cut-off value was defined as fold change > 2 or <  − 2, adjusted P < 0.05. B–D The expression level of hsa_circ_0050386 in lung cancer tissue from two regions in China. High expression was defined as Log2 (T/N expression) value > 0, and low expression was defined as Log2 (T/N expression) value < 0. N, nontumorous tissue; T, tumorous tissue. E The schematic showing hsa_circ_0050386 encoded from exon 17,18 of the ANDRD27 gene. F PCR and agarose gel electrophoresis detecting hsa_circ_0050386 and GAPDH in cDNA and gDNA by using convergent and divergent primers in A549 and PC9 cells. G PCR, and agarose gel electrophoresis detecting hsa_circ_0050386 by convergent and divergent primers in A549 and PC9 cells after treatment with RNase R. gDNA was used as a control. gDNA, genomic DNA. H Detection of subcellular location of hsa_circ_0050386 by FISH. Hsa_circ_0050386 probes were labeled with Cy3; Nuclei were stained with DAPI. I Detection of the cytoplasmic and nuclear distribution of hsa_circ_0050386 in A549 and PC9 cells by qRT-PCR. GAPDH and U1 were used as positive controls for cytoplasm and nucleus, respectively