Bladder cancer risk stratification with the Oncuria 10-plex bead-based urinalysis assay using three different Luminex xMAP instrumentation platforms

Table 4 Biomarker protein concentrations in urine samples compared across three analyzers (pg/mL)

Sample ID	Instrument	A1AT	ANG	ApoE	CA9	IL-8	MMP-9	MMP-10	PAI-1	SDC1	VEGF-A
#0003	MagPix	185,250	4320	10,566	49	1410	1610	709	4356	38,128	6225
Tumor	200	185,250	4320	11,668	44	1410	1652	720	4639	38,657	6545
	FlexMap 3D	185,250	4320	11,707	41	1410	1694	761	4649	40,278	6646
#0146	MagPix	185,250	1952	2618	11	855	161	13	1889	29,133	1496
Tumor	200	185,250	1922	2823	6	893	179	13	1832	27,662	1535
	FlexMap 3D	185,250	1978	2728	10	903	170	13	1914	29,256	1567
#0147	MagPix	185,250	479	204	1	138	36	13	103	11,336	50
Tumor	200	185,250	475	204	1	140	46	13	109	11,115	57
	FlexMap 3D	185,250	464	204	1	139	40	13	106	11,111	53
#0010	MagPix	3570	28	204	1	2	21	13	8	3099	25
Control	200	3872	25	204	4	2	21	13	8	3306	29
	FlexMap 3D	3581	28	204	1	2	21	13	8	3155	28
#0145	MagPix	48,207	71	1326	1	2	21	13	8	34,823	161
Control	200	49,254	73	1519	1	3	21	13	8	35,446	177
	FlexMap 3D	49,624	71	1408	2	3	21	13	8	34,831	166
#0150	MagPix	4288	12	204	1	2	21	13	8	8971	12
Control	200	4539	11	204	1	2	21	13	8	8725	12
	FlexMap 3D	4247	12	204	4	2	21	13	8	8898	12

Shown is output from six representative urine samples, from three confirmed BC subjects (“Tumor”) and three Control subjects. Values are averages of duplicate wells per analyte, per subject, rounded to the nearest whole number. Values that exceeded the highest standard curve concentration for any individual analyte were assigned that biomarker’s highest standard value. The full dataset for all 36 subjects is provided in Additional file 1: Table S1

ISSN: 1479-5876