Fig. 4

Hypothalamic neuronal activity under 5-HT_1Areceptor agonist treatment. After fluoxetine injection for 4 weeks, hypothalamic CRH neuronal activity (CRH, CRH_R2 and c-Fos) before and after challenge with 8-OH-DPAT was evaluated by immunofluorescent staining (A) and western blotting (B) analysis. In the hypothalamic plasma membrane, the protein expression levels of the 5-HT_1A receptors, GIRK1 and GIRK2 were measured (C). The protein expression levels of BDNF, phosphorylated ERK1/2 and CREB in whole hypothalamic lysate were analyzed (D). The data are expressed as the mean ± SD (n = 4/group)