Fig. 1

Experimental workflow. Illustration depicting the process from cancer surgery to gene expression analyses of the patient-derived scaffold (PDS) cultures. Primary cancer samples are decellularized to generate PDSs that are cryotome sectioned into 150 µm slices and then used for cancer cell line cultures. After 21 days of adaptation to the patient cancer microenvironment, the PDS cultures are treated with 5-fluorouracil (5-FU) or doxorubicin (DOX) for 48 h followed by gene expression analyses by quantitative PCR. Created with BioRender.com