Fig. 5

PIGT promotes GLUT1 glycosylation and membrane trafficking. A, B Expression of PIGT and GLUT1 in 253 J and T24 cells infected with shNC or PIGT shRNA. C Immunofluorescence for GLUT1 (green) in BIU-87 cells overexpressing PIGT (oePIGT) (scale bar, 50 μm). D FACS analysis CD55 membranous expression in BIU-87-oePIGT or BIU-87-vector. E Glycosylated and non-glycosylated GLUT1 in BIU-87-oePIGT or BIU-87-vector treated with tunicamycin (Tun). F GLUT1 expression levels in BIU-87 cells treated by cycloheximide. ***P < 0.001 vs shNC or vector