Fig. 4
WTAP promotes the m6A modification of PIGT through IGF2BP2. A The global m6A level in bladder cancer tumor and normal tissues of the hospital cohort (n = 25). B m6A levels of PIGT 3′-UTR in 253 J cells. C m6A levels of PIGT 3′-UTR in 253 J cells transfected with METTL3, METTL14 or WTAP siRNA. D PIGT expression in 253 J-siWTAP and in BIU-87-vector or BIU-87-oeWTAP. E PIGT expression in 253 J cells transfected with IGF2BP1, IGF2BP2 or IGF2BP3 siRNA. F IGF2BP2 knockdown in 253 J cells decreased PIGT mRNA stability. G Interaction between IGF2BP2 and PIGT 3′UTR. ***P < 0.001 vs normal or siNC. ###P < 0.001 vs vector