Fig. 3

PIGT knockdown inhibits cell metastasis. 253 J and T24 cells were infected with lentivirus containing shNC or PIGT shRNA (shRNA), and the A, B migration and C, D invasion were detected. 253 J cells infected with shNC or PIGT shRNA lentivirus were injected to mice. E Histology of metastasized lungs of mice (scale bar, 200 μm). F Quantification of the number of metastatic nodules (6 mice in each group). G Survival rates of mice (20 mice in each group). ***P < 0.001 vs shNC