Fig. 7
In vitro co-culture experiments revealed that ALOX5 recruited M2 macrophages. a WB showed the change of protein expression level after transfection of si-ALOX5. b WB showed the change of protein expression level after treatment of CCLP-1 cells with Zileuton. c WB demonstrated the change of protein expression level after transfection of ALOX5 overexpression plasmid in HUCCT-1 cells. d Transwell pattern diagram. e, f Transwell image displayed of the effect of conditioned medium on the migration and invasion of M2 macrophages. g, h Bar graph showing the effect of ICC-CM on M2 macrophage migration and invasion capacity after knockdown of ALOX5 or Zileuton treatment. i Bar graph showing the effect of ICC-CM on M2 macrophage migration capacity after overexpression of ALOX5. (*: P < 0.05, **: P < 0.01, ***: P < 0.001, ****: P < 0.0001)