Fig. 4

Expression level of ALOX5 was correlated with the abundance of M2 macrophage infiltration in ICC tissues. a Multiple immunofluorescence staining images demonstrating the abundance of M2 macrophage infiltration in regions with high ALOX5 expression. b Multiple immunofluorescence staining images demonstrating the abundance of M2 macrophage infiltration in regions with low ALOX5 expression. c Model diagram of multiplex immunofluorescence staining analysis of 12 ICC tissues. d Paired diagram of the number of M2 macrophages in ALOX5 high and low expression areas. (*P < 0.05, **: P < 0.01, ***: P < 0.001, ****: P < 0.0001)