Fig. 12
Infiltrating M2 macrophages promote ICC migration and invasion. a Wound healing test showed the effects of control, M0 and M2 macrophages on the migration ability of CCLP-1 cells. b Bar graph demonstrating wound healing rate for control and M2-CM treated CCLP-1 cells. c Wound healing test showed the effects of control, M0 and M2 macrophages on the migration ability of HUCCT-1 cells. d Bar graph showing wound healing rate for control and M2-CM treated HUCCT-1 cells. (e) Transwell images showing the effects of control, M0, and M2 macrophages on migration and invasion of CCLP-1 cells. f Histograms demonstrated the changes of migration and invasion ability of CCLP-1 cells in control group, M1-CM group and M2-CM group. g WB images showed the changes of EMT-related protein expression level in CCLP-1 cells after co-culture with control, M0 and M2 macrophages. h Transwell images showing the effects of control, M0, and M2 macrophages on migration and invasion of HUCCT-1 cells. i Histograms demonstrated the changes of migration and invasion ability of HUCCT-1 cells in control group, M1-CM group and M2-CM group. j WB images showed the changes of EMT-related protein expression level in HUCCT-1 cells after co-culture with control, M1 and M2 macrophages. (*: P < 0.05, **: P < 0.01, ***: P < 0.001, ****: P < 0.0001)