Fig. 3

IL20RB promotes stemness and chemoresistance in pancreatic cancer in vivo. A–B Tumor formation results after subcutaneous injection of 10⁵, 10⁶ and 10⁷ PANC-1, respectively, into nude mice. C Tumor growth curve (n = 5 per group). D Statistical analysis of tumor weight at the end of the trial (n = 5 per group). E–F Representative immunohistochemical (IHC) images of NANOG and SOX2 in tumor tissue sections and statistical analysis of IHC scores, scale bar = 40 µm. G Western blot analysis of NANOG, SOX2 and IL20RB in tumor tissues. (H) 10^7 PANC-1 was injected subcutaneously into nude mice, and then gemcitabine 100 mg/kg was intraperitoneally injected twice a week for 4 weeks. I Statistical analysis of tumor weight at the endpoint. J Tumor growth curve. K–L Representative IHC images of pan-CK in tumor tissue sections and statistical analysis of IHC scores, scale bar = 40 µm