Fig. 2

Rictor forms a mutual binding interaction with wild-type p53. A Coimmunoprecipitation (Co-IP) and immunoblotting (IB) in Hep3B cells 48 h after transfection with pCMV-p53^wt. B Confocal microscopy (CM) images of Rictor, p53, and mTOR in HCC cell lines. 1: Green Rictor staining (except column (b), showing green p53 staining); 2: Red p53 staining (except column (b), showing red mTOR staining); 3: Blue Hoechst nuclear staining; 4: Merged staining of 1 + 2 + 3; 5: Merged staining of 1 + 2; 6: Magnified regions are indicated by white arrows. C RT-qPCR analysis of Bax and p21 mRNA expression in Huh7 cells after 24 h of transfection with pCMV-p53^wt. An irrelevant miRNA pre-miR-375 served as a parallel control. D Western blotting assay and densitometric analysis in Huh7 cells (transfected with pCMV-p53^wt) 48 h after transfection. E RT-qPCR measurement of miR-192 expression levels in HCC cells 48 h after transfection. *P < 0.05; ns, not significant