Fig. 1
Rictor exhibits a p53wt-dependent HCC-promoting effect in vitro. A Western blotting assay and protein quantitative analysis in Huh7 cells: effect of pre-miR-192 on mTOR, Rictor, and Raptor levels. Pre-miR-NC was the negative control. Two unrelated microRNAs, pre-miR-885 and pre-miR-122, were used as parallel controls. B TargetScan-predicted miR-192 binding site at Rictor 3’UTR. Luciferase reporter assay showed that miR-192 significantly inhibited miR-192 on the reporter vectors but not in other vectors. Luciferase activity was standardized to that of β-Gal as a control. C, D Cell proliferation assessed by MTT assay 72 h after transfection with miR-192 precursors or siRNAs for Rictor (siRictor) in PLC/PRF/5 and Huh7 cells. MiR-NC and siNC were set as negative controls (NC). *P < 0.05; ns, not significant