Fig. 6

Erbin binds to TFEB and regulates the stability of TFEB-14-3-3 and TFEB-PPP3CB complexes. A 293T cells were transfected with indicated plasmids. 48 h post-transfection, Co-IP and immunoblot analysis were performed with indicated antibodies. B, C Experiments were performed similarly to those in A, except indicated plasmids were used. D Schematic diagram of the full-length and truncated constructs of Erbin. The 293T cells were co-transfected with Myc-TFEB and the indicated truncated Erbin constructs for 48 h. Co-immunoprecipitation and immunoblot analyses were performed with the indicated antibodies. E Schematic diagram of the full-length and truncated constructs of TFEB. The 293T cells were co-transfected with HA-Erbin and the indicated truncated TFEB constructs for 48 h. Co-immunoprecipitation and immunoblot analyses were performed with the indicated antibodies