Fig. 4
Erbin enhanced lysosome biogenesis in BMDMs. A BMDMs were treated with 10 μg/ml MDP (6 h) and then stained with LysoTracker Red for 15 min. The fluorescence intensity of treated cells was measured by confocal microscopy. Scale bar: 25 μm. B Fluorescence puncta were quantified by analysis of results of (A). C Erbinfl/fl and Erbinfl/fl/Lyz2−cre BMDMs were treated with MDP for 6 h, and western blotting was used to estimate the protein levels of LAMP2, CTSB, ATP6V1A, and ATP6V1B2. *P < 0.05, **P < 0.01, ***P < 0.001 from ANOVA followed by Tukey’s post hoc test