Fig. 7

ISOX exerts the anti-osteosarcoma effect. A Schematic showing steps for identifying potential anti-osteosarcoma drugs base on CMap database. B Molecular structural formulas of small molecular compounds with CMap scores less than − 90. C, D IC50 of five small molecular compounds in 143B and SJSA-1 cells according to the CCK-8 assay. E Cell proliferation, F colony formation, G migration and invasion abilities were assessed in ISOX-treated 143B and SJSA-1 cells. H, I, J Tumor volume, tumor weight and the number of lung metastasis nodules in BALB/C nude mouse tibial orthotopic models with ISOX 50 mg/kg/daily and 100 mg/kg/daily treatment (n = 5 per group). K, L, M General view, tumor volume, tumor weight in K7M2 syngeneic tumors with ISOX 100 mg/kg/daily treatment (n = 5 per group). N, O CD8+ T cells, G-MDSC, M-MDSC, macrophage and CD8+ T cell functional markers analysed by flow cytometry. *p < 0.05, **p < 0.01, ***p < 0.001