Fig. 4

STC2 correlates with poor prognosis and promotes osteosarcoma proliferation and metastasis in vitro. A Kaplan–Meier survival analysis of STC2 in TARGET-OS cohort. B IHC staining of STC2 in 62 paraffin-embedded osteosarcoma tissues. Scale bar: 50 μm (left) and 100 μm (right). C, D Kaplan–Meier survival analysis of osteosarcoma patients stratified by STC2 expression level (n = 62). E Protein levels of STC2 in HFOB1.19 cells and several osteosarcoma cell lines by Western blot analysis. F Knockdown of STC2 in 143B and SJSA-1 osteosarcoma cells. G Cell proliferation, H colony formation, I, J Migration and invasion ability of STC2 knockdown 143B and SJSA-1 cells. *p < 0.05, **p < 0.01, ***p < 0.001