Fig. 6

EZH2 is a direct transcriptional target of the transcription factor SOX4. A Sequence logo of SOX4. B Potential binding sites of SOX4 at the promoter of EZH2. C Correlation between SOX4 and EZH2 expression levels. The mRNA levels of SOX4 were correlated with EZH2 (r = 0.53; P = 0.0026). D Relative enrichment of SOX4 on the promoter region of EZH2 gene was evaluated by CHIP-qPCR in HK-2 cell. E The effect of SOX4 knockdown on lipid peroxidation. F Relative lipid peroxidation, MDA, GSH, and Fe2 + levels. G Western blotting analysis of SOX4, EZH2, SLC7A11, and GPX4. Scale bar = 50 µm. *P < 0.05 and **P < 0.01 compared with the siNC group; ^#P < 0.05 and ^##P < 0.01 compared with the Ox + siNC group