Fig. 5

EZH2 regulates ferroptosis by directly suppressing SLC7A11 expression. A Heat map showed the genes significantly upregulated in the shEZH2 group. Red and blue colors denote ferroptosis-related upregulated and downregulated genes, respectively. B Western blotting analysis of H3K27me3. C Genome browser view of the H3K27me3 density at the SLC7A11 promoter region. D Analysis of EZH2-SLC7A11 promoter interaction using the CHIP assay. E SLC7A11 inhibition reverses the effects of the EZH2 knockdown on ferroptosis. The lipid ROS levels were analyzed using the C11-BODIPY probe. F Western blotting analysis of EZH2, SLC7A11, and GPX4. **P < 0.01, ***P < 0.001 compared with the shNC group; ^###P < 0.001 compared with the Ox + shNC group in B. *P < 0.05 and **P < 0.01 compared with the Ox group, ^#P < 0.05 and ^##P < 0.01 compared with the Ox + shEZH2 group in F