Fig. 4

EZH2 knockdown mitigates oxalate-induced ferroptosis in HK-2 cells. A The effect of EZH2 knockdown on lipid peroxidation in vitro. B Effects of EZH2 knockdown on lipid ROS levels were analyzed using the C11-BODIPY fluorescence probe. C Immunofluorescence analysis of SLC7A11, GPX4, and ACSL4. D Relative lipid peroxidation, MDA, GSH, and Fe2 + levels in HK-2 cells. E Western blotting analysis of EZH2, SLC7A11, GPX4, ACSL4 and PTGS2. F The mRNA levels of EZH2, SLC7A11, GPX4, ACSL4 and PTGS2. Scale bar = 50 µm. *P < 0.05, **P < 0.01, and ***P < 0.001 compared with the shNC group; ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 compared with the Ox + shNC group