Fig. 3

EZH2 knockout alleviates CaOx-induced kidney injury and ferroptosis. A Diagram of mouse moulding. B Effect of EZH2 knockout on bodyweight (n = 6). C H&E and Von-Kossa staining (n = 6). D Serum BUN and Cr levels in different groups (n = 6). E Immunofluorescence analysis of SLC7A11, GPX4, and ACSL4 in kidney tissues (n = 6). F Representative images were acquired using a TEM. G Western blotting analysis of EZH2, SLC7A11, GPX4, ACSL4 and PTGS2. H Relative MDA, GSH, and Fe2 + levels (n = 6). I EZH2, SLC7A11, GPX4, and ACSL4 mRNA levels were testing using qRT-PCR. Scale bar = 50 µm. *P < 0.05, **P < 0.01, and ***P < 0.001 compared with the EZH2^fl/fl group; ^#P < 0.05, ^##P < 0.01, and ^###P < 0.01 compared with the Gly + EZH2^fl/fl group