Fig. 2

EZH2 is upregulated in kidney stone patients, kidney stone mice, and oxalate-stimulated HK-2 cells. A Single-nucleus RNA sequencing dataset analysis of CKD patients and healthy controls. B Volcano map of the DEGs between the Gly and control groups. C Western blotting quantitative analysis of EZH2 in vivo and in vitro. D Expression levels of EZH2 in kidney stone patients, kidney stone mice, and the control groups were examined using immunohistochemical analysis. E Immunofluorescence analysis of EZH2. Scale bar = 50 µm. *P < 0.05 and **P < 0.01 compared with the control group