Table 1 Antibodies
Antibody | Source | Vendor | Catalog no. | Dilution factor |
|---|---|---|---|---|
Sirt1 | Rabbit | Proteintech | 13161-1-AP | 1:1000 |
Pgc-1α | Mouse | Proteintech | 66369-1-Ig | 1:1000 |
FoxO3a | Rabbit | Cell Signaling Technology | #2497 | 1:1000 |
p-FoxO3a (Ser253) | Rabbit | Cell Signaling Technology | #9466 | 1:500 |
Acetylated-Lysine | Rabbit | Cell Signaling Technology | #9441 | 1:500 |
Bax | Rabbit | Cell Signaling Technology | #2772 | 1:1000 |
Bcl-2 | Mouse | ImmunoWay | YM3041 | 1:1000 |
caspase-3 | Rabbit | Cell Signaling Technology | #9662 | 1:1000 |
cleaved caspase-3 (Asp175) | Rabbit | Cell Signaling Technology | #9661 | 1:1000 |
PARP | Rabbit | Cell Signaling Technology | #9532 | 1:1000 |
Cleaved-PARP | Rabbit | Cell Signaling Technology | #94885 | 1:500 |
LC3A/B | Rabbit | Cell Signaling Technology | #4108 | 1:1000 |
Beclin1 | Rabbit | Cell Signaling Technology | #3495 | 1:1000 |
p62 | Rabbit | ABcam | ab91526 | 1:1000 |
ATG5 | Rabbit | Proteintech | 10181-2-ap | 1:1000 |
PLIN2 | Rabbit | Proteintech | 15294-1-AP | 1:1000 |
LAMP1 | Mouse | Cell Signaling Technology | 15665 | 1:1000 |
NRF1 | Rabbit | Proteintech | 12482-1-AP | 1:1000 |
TFAM | Rabbit | Proteintech | 22586-1-AP | 1:1000 |
NDUFB8 | Rabbit | Proteintech | 14794-1-AP | 1:1000 |
MT-CO2 | Rabbit | Cell Signaling Technology | #31219 | 1:1000 |
SDHB | Rabbit | Cell Signaling Technology | #92649 | 1:1000 |
HSP90 | Rabbit | Proteintech | 13171-1-AP | 1:5000 |
β-Tubulin | Rabbit | Proteintech | 10068-1-AP | 1:10,000 |