Fig. 5

Didymin suppresses apoptosis by activating Sirt1 in PA-treated AML12 cells. A Representative images of TUNEL staining in AML12 cells (Scale bar = 100 μm). Quantification of the percentage of TUNEL-positive cells (n = 3). B Apoptosis analysis of AML12 cells by flow cytometry. And the results of quantitative analyses of apoptosis rate (n = 3). C Western blot analysis of Bax, Bcl2, cleaved-caspase3, and caspase3 proteins in AML12 cells (n = 3). Data are expressed as mean ± SD. *P < 0.05, ***P < 0.001 PA vs. PA + Didymin. # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 control vs. PA. PA palmitic acid, TUNEL TdT-mediated dUTP Nick-End labeling, Bax Bcl-2-associated X protein, Bcl2 B-cell lymphoma 2