Fig. 4

Didymin enhances lipophagy by activating Sirt1 in PA-treated AML12 cells. A AML12 cells transfected with mCherry-eGFP-PLIN2 plasmid. (Scale bar = 30 μm). B Immunoprecipitation of FoxO3a in AML12 cells showing acetylated FoxO3a level (n = 3). C Western blot analysis of LC3, Beclin1, P62, PLIN2, LAMP1 and ATG5 in AML12 cells (n = 3). Data are expressed as mean ± SD. *P < 0.05, **P < 0.01 PA vs. PA + Didymin. ^##P < 0.01, ^###P < 0.001, ^####P < 0.0001 control vs. PA. PA palmitic acid, FoxO3a Foxkhead Box Class O 3a, PLIN2 perilipin 2, LAMP1 lysosomal associated membrane protein 1