Fig. 3

Myelin water fraction (MWF) according to the proteolipid protein (PLP) immunoreactive area value and age. MWF showed significant association with PLP immunoreactive area value in the corpus callosum (A, left) and cortex (A, middle) in all C57BL/6 mice and in seven 12-week-old C57BL/6 mice (A, right). MWF showed significant association with age in the corpus callosum (B, left) and cortex (B, right). Representative PLP immunoreactive staining images (left column) and MWF maps (right column) of C57BL/6 mice of different ages (C). Solid lines indicate the linear regression lines of best fit, and dashed lines indicate the 95% confidence intervals