Fig. 3

Subretinal transplantation of hMSCs, hiPSCs, and hiPSC-RPE cells. A The procedure for cell labeling with lentivirus-GFP. B The cell morphology (i, vi) and green fluorescent protein (GFP) expression of hAFSCs (i–iii) and hDPSCs (vi–viii) after lentivirus-GFP transduction. (iii) and (viii) were generated by merging (i, ii) and (vi, vii), respectively. Scale bar: 100 μm. Bright field (iv, ix) and fluorescent channel images (v, x) of fundus photographs of the eyes of RCS rats after subretinal injection of lentivirus-GFP-labeled hAFSCs (iv, v) and hDPSCs (ix, x), where the green fluorescence was hard to find on the fundus photograph of rats subjected to lentivirus-GFP labeled cells. Scale bar: 600 μm. C The procedure for cell labeling with CellTracker. D Cell pellets labeled with CellTracker on ice. E The cell morphology of different cells (i–vi) and fundus photographs (vii-xx) of subretinal injection of different cells and PBS into RCS rats, analyzed in bright field (vii-xiii) and fluorescent channels (xiv–xx) using hiPSCs (i, vii, xiv), hiPSC-RPE cells (ii, viii, xv), hDPSCs (iii, ix, xvi), hBMSCs (iv, x, xvii), hAFSCs (v, xi, xviii), hADSCs (vi, xii, xix), and (g) PBS (xiii, xx), where the fundus photographs of successful cell transplantation were characterized by the obvious green fluorescence within the well-defined retinal eminence, whereas the fundus photograph of control group subjected to PBS showed no fluorescence