Fig. 6

Expression of CD271 on SN-MM cells and co-expression of CD271 and ZEB1 on parental tumors. A CD271 flow cytometry analysis on SN-MM cell lines. CD271 expression is reported on X axes; on y-axes side scatter (SSC) is reported. Grey dot plots are negative controls. Dot plots are representative of two independent experiments. B Representative images of immunohistochemistry analysis of parental tumor biopsies, stained as indicated. Green rectangles mark cells co-expressing ZEB1 and CD271 and lacking MITF and CDH1. Black arrows point cells co-expressing MITF and CDH1 and lacking ZEB1 and CD271. Magnification 400x, scale bar 25 µm