Fig. 13

a. Retina immunofluorescence staining for EGR4 (red), GS (green), DAPI (blue), and overlay picture.EGR4 was identified to express on Müller cells. b. Differences in EGR4 expression in the retinal tissues of mice in different groups. EGR4 showed a tendency of upregulation accompanied with AdipoR1 after AdipoRon gavage. c. Strips quantified with Image J software. Results are shown as mean ± standard deviation, ***p < 0.001. EGR4, early growth response factor 4; GS, glutamine synthetase; DAPI, 4ʹ,6-diamidino-2-phenylindole; HFD, high-fat diet; DM, high-fat diet + diabetes mellitus control; DMSO, high-fat diet + diabetes mellitus + dimethyl sulfoxide negative control; AdipoRon, high-fat diet + diabetes mellitus + AdipoRon