Fig. 2

Effects of melatonin on T-cell activation in vitro. A Flow cytometry analysis of CD25 and CD69 expression in activated T cells treated with melatonin, as well as in control naïve T cells. B ELISA analysis of interferon-gamma (IFN-γ) and IL-2 production in melatonin-treated and control groups. C T-cell proliferation assays were conducted by pulse-labeling the stimulated T cells with [3H]thymidine for 8 h. Thymidine incorporation was measured as an indicator of cell proliferation. Data are representative of 3 independent experiments. Summary graphs (N = 4) are presented as mean ± SEM, and P values were determined by unpaired two-tailed Student’s t-test