Fig. 3

Identification of chemical constituents of broccoli extract OE, pre- and post-treatment with S9 fraction. A HPLC chromatograms recorded under UV at 320 nm show component peaks (numbered 1–14) in the broccoli extracts pre- (undigested OE) and post-treatment (S9). Peaks: (1) Kaempferol-sinapoyl-tetrahexoside; (2) Kaempferol-sinapoyl-trihexoside; (3) Sinapoyl-hexoside; (4) Neoglucobrassicin; (5) Spermidine conjugate; (6) Sinapic acid; (7) Sinapoyl malate; (8) Disinapoyl-dihexoside; (9) Kaempferol-disinapoyl-trihexoside; (10) Disinapoyl-dihexoside (isomer of 8); (11) Disinapoyl-hexoside; (12) Trisinapoyl-dihexoside; (13) Trisinapoyl-dihexoside (isomer of 12); (14) Methyl sinapate. B Chemical composition of broccoli extract pre-and post-treatment with S9 fraction. The percentage area under the peaks in the HPLC chromatograms was used to quantitatively compare the compositions of the pre-and post-treatment extracts shown as bar charts and with identities color-coded at right of the bar charts, with compound identity listed on the right side of each bar. The fifteenth bar at the bottom of the chart represents other cumulative small molecules